Genipin-mediated immobilization of glucose oxidase enzyme on carbon felt for use as heterogeneous catalyst in sustainable wastewater treatment

Enzyme immobilization is necessary process to improve the bioactivity and stability of biocatalyst. In this study, glucose oxidase (GOx) enzyme was immobilized on plasma-treated fibrous carbon felt as a textile carrier produce heterogeneous catalyst. Genipin, naturally occurring crosslinker, that has less cytotoxicity than conventional crosslinkers, used in process. UV-Vis FTIR spectra confirmed crosslinking reaction between genipin primary amines GOx enzyme, by forming blue-pigmented aggregates. relative activity after maintained up 40%, with performance 6 cycles for plasma treated carbon, while maintaining their bio-electro-activity shown from cyclic voltammetry scans (CV). The obtained catalysts have been tested use sustainable wastewater treatment Remazol Blue RR (RB) dyestuff means Bio-Fenton (BF) enzymatic Bio-electro-Fenton (BEF) processes. produced samples resulted high color removal efficiency, 93% discoloration first 3 h. Meanwhile, 34% COD removal, simultaneous power density generation 0.16 ± 0.01 ?W.cm?2 at current around 10 2 ?A.cm?2 12 These results highlight importance bio-based crosslinker enzymes, potential both approaches step towards zero-energy degradation organic matter.